Transcriptome analysis of the induction of somatic embryogenesis in Coffea canephora and the participation of ARF and Aux/IAA genes

PeerJ

article

Ana O. Quintana-Escobar¹  Geovanny I. Nic-Can²  Rosa María Galaz Avalos¹  Víctor M. Loyola-Vargas¹  Elsa Gongora-Castillo³ 

[1] Unidad de Bioquímica y Biologia Molecular de Plantas;CONACYT Research Fellow-Facultad de Ingeniería Química, Universidad Autónoma de Yucatán;CONACYT Research Fellow-Unidad de Biotecnología

关键词: Auxin response;    Cellular differentiation;    Growth regulators;    Somatic embryogenesis;    Signaling;    Cellular totipotency;   

DOI  :  10.7717/peerj.7752

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundSomatic embryogenesis (SE) is a useful biotechnological tool to study the morpho-physiological, biochemical and molecular processes during the development of Coffea canephora. Plant growth regulators (PGR) play a key role during cell differentiation in SE. The Auxin-response-factor (ARF) and Auxin/Indole-3-acetic acid (Aux/IAA) are fundamental components involved in the signaling of the IAA. The IAA signaling pathway activates or represses the expression of genes responsive to auxins during the embryogenic transition of the somatic cells. The growing development of new generation sequencing technologies (NGS), as well as bioinformatics tools, has allowed us to broaden the landscape of SE study of various plant species and identify the genes directly involved.MethodsAnalysis of transcriptome expression profiles of the C. canephora genome and the identification of a particular set of differentially expressed genes (DEG) during SE are described in this study.ResultsA total of eight ARF and seven Aux/IAA differentially expressed genes were identified during the different stages of the SE induction process. The quantitative expression analysis showed that ARF18 and ARF5 genes are highly expressed after 21 days of the SE induction, while Aux/IAA7 and Aux/IAA12 genes are repressed.DiscussionThe results of this study allow a better understanding of the genes involved in the auxin signaling pathway as well as their expression profiles during the SE process.

【 授权许可】

CC BY