期刊论文详细信息
PeerJ
Genome size, chromosome number determination, and analysis of the repetitive elements in Cissus quadrangularis
article
Duncan Kiragu Gichuki1  Lu Ma4  Zhenfei Zhu1  Chang Du1  Qingyun Li1  Guangwan Hu1  Zhixiang Zhong1  Honglin Li1  Qingfeng Wang1  Haiping Xin1 
[1] CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences;Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences;University of Chinese Academy of Sciences;Shenzhen Tobeacon Technology Co. Ltd.
关键词: Cissus quadrangularis;    Genome size;    Copia;    Repeat elements;    Chromosome counts;    Gypsy;    Flow cytometry;    C value;   
DOI  :  10.7717/peerj.8201
学科分类:社会科学、人文和艺术(综合)
来源: Inra
PDF
【 摘 要 】

Cissus quadrangularis (Vitaceae) is a perennial climber endemic to Africa and is characterized by succulent angular stems. The plant grows in arid and semi-arid regions of Africa especially in the African savanna. The stem of C. quadrangularis has a wide range of applications in both human and animal medicine, but there is limited cytogenetic information available for this species. In this study, the chromosome number, genome size, and genome composition for C. quadrangularis were determined. Flow cytometry results indicated that the genome size of C. quadrangularis is approximately 2C = 1.410 pg. Fluorescence microscopy combined with DAPI stain showed the chromosome numbers to be 2n = 48. It is likely that C. quadrangularis has a tetraploid genome after considering the basic chromosome numbers in Cissus genus (n = 10, 11, or 12). A combination of low-throughput genome sequencing and bioinformatics analysis allowed identification and quantification of repetitive elements that make up about 52% of the C. quadrangularis genome, which was dominated by LTR-retrotransposons. Two LTR superfamilies were identified as Copia and Gypsy, with 24% and 15% of the annotated clusters, respectively. The comparison of repeat elements for C. quadrangularis, Vitis vinifera, and four other selected members in the Cissus genus revealed a high diversity in the repetitive element components, which could suggest recent amplification events in the Cissus genus. Our data provides a platform for further studies on the phylogeny and karyotype evolution in this genus and in the family Vitaceae.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO202307100009122ZK.pdf 2260KB PDF download
  文献评价指标  
  下载次数:6次 浏览次数:1次