Validity and reliability evidence of a point of care assessment of salivary cortisol and α -amylase: a pre-registered study

PeerJ

article

Kagan J. Ducker¹  Robin L.J. Lines¹  Michael T. Chapman¹  Peter Peeling²  Alannah K.A. McKay²  Daniel F. Gucciardi¹ 

[1] School of Physiotherapy and Exercise Science, Curtin University;School of Human Sciences ,(Exercise and Sport Science), The University of Western Australia;Australian Institute of Sport

关键词: Stress;    Psychophysiology;    Saliva;    Exercise;   

DOI  :  10.7717/peerj.8366

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

PurposeThe iPro Cube is a small portable point-of-care device designed to analyse salivary markers of stress in a user-friendly manner (e.g., fast, convenient). Our aim was to test the reliability and validity of the iPro Cube to measure salivary cortisol and α-amylase as compared to the common laboratory standard method (ELISA immunoassay) prior to and after moderate intensity exercise.MethodsThe study was a repeated measures, pre-registered design, and statistical framework that incorporated prior knowledge directly into the estimation process. Twenty-nine individuals (age = 27.4 ± 6.6 y; body-mass = 70.8 ± 11.3 kg; height = 1.74 ± 0.92 m; 18 males) completed a single PWC75%HRmax, with repeated measures of salivary cortisol and -amylase pre, immediately post, and 30 min post-exercise.Resultsr r r r < 0.77).ConclusionsThe iPro Cube is capable of taking measures of salivary cortisol that are moderately correlated to values obtained via ELISA immunoassay, however the unit underestimates salivary cortisol and overestimates salivary -amylase at rest and post-moderate intensity exercise. It is recommended that researchers continue using standard laboratory techniques to assess these salivary stress markers.

【 授权许可】

CC BY