Postharvest biochemical characteristics and ultrastructure of Coprinus comatus

PeerJ

article

Yi Peng¹  Tongling Li²  Huaming Jiang³  Yunfu Gu¹  Qiang Chen¹  Cairong Yang²  Wei liang Qi²  Song-qing Liu²  Xiaoping Zhang¹ 

[1] College of Resources, Sichuan Agricultural Uniersity;College of Chemistry and Life Sciences, Chengdu Normal University;Sichuan Vocational and Technical College;Institute of Microbiology, Chengdu Normal University

关键词: Chicken drumstick mushroom;    Postharvest storage;    Cell degradation;    TEM;    Browning;    SOD;    POD;    CAT;    Glucanase;    PG;   

DOI  :  10.7717/peerj.8508

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCoprinus comatus is a novel cultivated edible fungus, hailed as a new preeminent breed of mushroom. However, C. comatus is difficult to keep fresh at room temperature after harvest due to high respiration, browning, self-dissolve and lack of physical protection.MethodsIn order to extend the shelf life of C. comatus and reduce its loss in storage, changes in quality, biochemical content, cell wall metabolism and ultrastructure of C. comatus (C.c77) under 4 °C and 90% RH storage regimes were investigated in this study.ResultsThe results showed that: (1) After 10 days of storage, mushrooms appeared acutely browning, cap opening and flowing black juice, rendering the mushrooms commercially unacceptable. (2) The activity of SOD, CAT, POD gradually increased, peaked at the day 10, up to 31.62 U g−1 FW, 16.51 U g−1 FW, 0.33 U g−1 FW, respectively. High SOD, CAT, POD activity could be beneficial in protecting cells from ROS-induced injuries, alleviating lipid peroxidation and stabilizing membrane integrity. (3) The activities of chitinase, β-1,3-glucanase were significantly increased. Higher degrees of cell wall degradation observed during storage might be due to those enzymes’ high activities. (4) The fresh C. comatus had dense tissue and every single cell had the number of intracellular organelles which structure can be observed clearly. After 10 d storage, the number of intracellular organelles was declined and the structure was fuzzy, the nucleus disappeared. After 20 d storage, C. comatus’s organization was completely lost, many cells were stacked together and the cell wall was badly damaged.

【 授权许可】

CC BY