Fluid resuscitation-related coagulation impairment in a porcine hemorrhagic shock model

PeerJ

article

Alexander Ziebart¹  Robert Ruemmler¹  Christian Möllmann¹  Jens Kamuf¹  Andreas Garcia-Bardon¹  Serge C. Thal¹  Erik K. Hartmann¹ 

[1] Department of Anesthesiology, Medical Centre of the Johannes Gutenberg-University

关键词: Gelatine-polysuccinate;    HES;    Hydroxyethyl starch;    Hemorrhagic shock;    Thromboelastometry;    ROTEM;    Pig model;    Blood;    Fluid resuscitation;   

DOI  :  10.7717/peerj.8399

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundFast and effective treatment of hemorrhagic shock is one of the most important preclinical trauma care tasks e.g., in combat casualties in avoiding severe end-organ damage or death. In scenarios without immediate availability of blood products, alternate regimens of fluid resuscitation represent the only possibility of maintaining sufficient circulation and regaining adequate end-organ oxygen supply. However, the fluid choice alone may affect the extent of the bleeding by interfering with coagulation pathways. This study investigates the impact of hydroxyethyl starch (HES), gelatine-polysuccinate (GP) and balanced electrolyte solution (BES) as commonly used agents for fluid resuscitation on coagulation using a porcine hemorrhagic shock model.MethodsFollowing approval by the State and Institutional Animal Care Committee, life-threatening hemorrhagic shock was induced via arterial blood withdrawal in 24 anesthetized pigs. Isovolumetric fluid resuscitation with either HES, GP or BES (n = 3 × 8) was performed to compensate for the blood loss. Over four hours, hemodynamics, laboratory parameters and rotational thromboelastometry-derived coagulation were analyzed. As secondary endpoint the porcine values were compared to human blood.ResultsAll the agents used for fluid resuscitation significantly affected coagulation. We measured a restriction of laboratory parameters, clot development and clot firmness, particularly in HES- and GP-treated animals. Hemoglobin content dropped in all groups but showed a more pronounced decline in colloid-treated pigs. This effect was not maintained over the four-hour monitoring period.ConclusionHES, GP, and BEL sufficiently stabilized the macrocirculation, but significantly affected coagulation. These effects were most pronounced after colloid and particularly HES administration. Despite suitability for rapid hemodynamic stabilization, colloids have to be chosen with caution, because their molecular properties may affect coagulation directly and as a consequence of pronounced hemodilution. Our comparison of porcine and human coagulation showed increased coagulation activity in pig blood.

【 授权许可】

CC BY