Identification of prognostic splicing factors and exploration of their potential regulatory mechanisms in pancreatic adenocarcinoma

PeerJ

article

Min-hua Rong¹  Zhan-hui Zhu¹  Ying Guan²  Mei-wei Li¹  Jia-shuo Zheng¹  Yue-qi Huang¹  Dan-ming Wei³  Ying-mei Li³  Xiao-ju Wu³  Hui-ping Bu¹  Hui-liu Peng¹  Xiao-lin Wei¹  Guo-sheng Li¹  Ming-xuan Li¹  Ming-hui Chen¹  Su-ning Huang² 

[1] Affiliated Cancer Hospital, Guangxi Medical University, Research Department;Affiliated Cancer Hospital, Guangxi Medical University, Department of Radiotherapy;First Affiliated Hospital, Guangxi Medical University, Department of Pathology

关键词: Splicing factors;    Pancreatic adenocarcinoma;    RNA-sequencing;    Overall survival;   

DOI  :  10.7717/peerj.8380

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Pancreatic adenocarcinoma (PAAD), the most common subtype of pancreatic cancer, is a highly lethal disease. In this study, we integrated the expression profiles of splicing factors (SFs) of PAAD from RNA-sequencing data to provide a comprehensive view of the clinical significance of SFs. A prognostic index (PI) based on SFs was developed using the least absolute shrinkage and selection operator (LASSO) COX analysis. The PI exhibited excellent performance in predicting the status of overall survival of PAAD patients. We also used the percent spliced in (PSI) value obtained from SpliceSeq software to quantify different types of alternative splicing (AS). The prognostic value of AS events was explored using univariate COX and LASSO COX analyses; AS-based PIs were also proposed. The integration of prognosis-associated SFs and AS events suggested the potential regulatory mechanisms of splicing processes in PAAD. This study defined the markedly clinical significance of SFs and provided novel insight into their potential regulatory mechanisms.

【 授权许可】

CC BY