The expression of small RNAs in exosomes of follicular fluid altered in human polycystic ovarian syndrome

PeerJ

article

Junhe Hu¹  Tao Tang¹  Zhi Zeng¹  Juan Wu¹  Xiansheng Tan¹  Jiao Yan¹ 

[1] Agriculture and Biotechnology Department, Hunan University of Humanities, Science and Technology, Loudi, Hunan province

关键词: Exosomes;    Human Follicular Fluid (HFF);    Polycystic ovary syndrome (PCOS);    PiRNAs;    MiRNA;   

DOI  :  10.7717/peerj.8640

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Polycystic ovary syndrome (PCOS) can cause reproductive disorders that may affect oocyte quality from punctured follicles in human follicular fluid (HFF). The non-coding RNA family includes micro RNA (miRNA), piwi-interacting RNA (piRNA) and transfer RNA (tRNA); these non-coding RNA transcripts play diverse functions and are implicated in a variety of diseases and health conditions, including infertility. In this study, to explore the role of HFF exosomes in PCOS, we extracted and sequenced RNA from HFF exosomes of PCOS patients and compared the analysis results with those of non-PCOS control group. The HFF exosomes were successfully isolated and characterized in a variety of ways. The sequencing results of the HFF exosomal RNA showed that about 6.6% of valid reads in the PCOS group and 8.6% in the non-PCOS group were successfully mapped to the human RNA database. Using a hierarchical clustering method, we found there were ten small RNA sequences whose expression was significantly different between the PCOS and non-PCOS groups. We chose six of them to predict target genes of interest for further GO analysis, and pathway analysis showed that the target genes are mainly involved in biosynthesis of amino acids, glycine, serine and glycosaminoglycan, as well as threonine metabolism. Therefore, the small RNA sequences contained in HFF EXs may play a key role in the mechanism that drives PCOS pathogenesis, and thereby can act as molecular biomarkers for PCOS diagnosis in the future.

【 授权许可】

CC BY