Diversity of fall armyworm, Spodoptera frugiperda and their gut bacterial community in Kenya

PeerJ

article

Joseph Gichuhi¹  Subramanian Sevgan¹  Fathiya Khamis¹  Johnnie Van den Berg²  Hannalene du Plessis²  Sunday Ekesi¹  Jeremy K. Herren¹ 

[1] International Centre of Insect Physiology and Ecology;Unit for Environmental Sciences and Management, North-West University;MRC-University of Glasgow Centre for Virus Research

关键词: Fall armyworm;    Spodoptera frugiperda;    Gut bacteria;    Corn strain;    Rice strain;    mtCOI haplotype;   

DOI  :  10.7717/peerj.8701

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. frugiperda could play a role in the insects’ success and adaptability. However, bacterial communities in S. frugiperda remain poorly studied. Methods We investigated the composition, abundance and diversity of microbiomes associated with larval and adult specimens of S. frugiperda collected from four maize growing regions in Kenya through high throughput sequencing of the bacterial 16S rRNA gene. The population structure of S. frugiperda in Kenya was assessed through amplification of the mitochondrial cytochrome oxidase subunit I gene. Results We identified Proteobacteria and Firmicutes as the most dominant bacterial phyla and lesser proportions of Bacteroidetes and Actinobacteria. We also observed differences in bacterial microbiome diversity between larvae and adults that are a likely indication that some prominent larval bacterial groups are lost during metamorphosis. However, several bacterial groups were found in both adults and larvae suggesting that they are transmitted across developmental stages. Reads corresponding to several known entomopathogenic bacterial clades as well as the fungal entomopathogen, Metarhizium rileyi, were observed. Mitochondrial DNA haplotyping of the S. frugiperda population in Kenya indicated the presence of both “Rice” and “Corn” strains, with a higher prevalence of the “Rice” strain.

【 授权许可】

CC BY