The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia

PeerJ

article

Xiu Liu¹  Qiulan Lv²  Hongyan Ren³  Liu Gao⁴  Peng Zhao²  Xiaomin Yang²  Guanpin Yang⁵  Daxing Xu¹  Guangtao Wang²  Wan Yang¹  Pengjun Wang²  Zenglan Wang¹  Shichao Xing¹ 

[1] Department of Pathogenic Biology, School of Basic Medicine, Qingdao University;Affiliated Hospital of Qingdao University;Shanghai Itechgene Technology Co.,Ltd;Medical Department of 3SBio Group;The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China;Institute of Sports Medicine and Health;School of Cardiovascular Medicine and Science, King’s College London

关键词: Gut microbiota;    Hyperuricemia;    Correlation;    Change;    Gut microbiota disorder;    Fecal microbiota transplantation;   

DOI  :  10.7717/peerj.8664

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target.

【 授权许可】

CC BY