Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

PeerJ

article

Bo Zhu¹  Jianjun Wu¹  Qingyong Ji²  Wei Wu¹  Shihui Dong¹  Jiayan Yu¹  Qiaoyan Zhang¹  Luping Qin¹ 

[1] School of Pharmacy, Zhejiang Chinese Medical University;Lishui Academy of Agricultural and Forestry Sciences

关键词: Continuous cropping;    Atractylodes macrocephala;    Fungal endophytes;    Rhizosphere fungi;    Fusarium;   

DOI  :  10.7717/peerj.8905

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Rhizospheric and endophytic fungi are key factors which influence plant fitness and soil fertility. Atractylodes macrocephala is one of the best-known perennial herbs used in traditional Chinese medicine. Continuous cropping has been shown to have a negative effect on its growth and renders it more susceptible to microbial pathogen attacks. In this study, we investigated the effects of continuous cropping on the endophytic and rhizospheric fungi associated with A.  macrocephala using culture-independent Illumina MiSeq. Continuous cropping was found to decrease fungal diversity inside plant roots, stems, leaves and tubers. Additionally, we found that the structure and diversity of rhizospheric and endophytic fungal communities were altered by root-rot disease. Fusarium was overrepresented among root-rot rhizospheric and endophytic fungi, indicating that it has a major negative impact on plant health during A.  macrocephala monocropping. Canonical correspondence analysis of the control and diseased samples revealed that pH, hydrolysis N, electrical conductivity and Hg content were well-correlated with fungal community composition during continuous cropping. Taken together, these results highlight the ecological significance of fungal communities in maintaining plant fitness and will guide the development strategies to attenuate the negative impacts of A.  macrocephala continuous cropping.

【 授权许可】

CC BY