Application of 16S rRNA gene sequencing in Helicobacter pylori detection

PeerJ

article

Aleksander Szymczak¹  Stanisław Ferenc²  Joanna Majewska¹  Paulina Miernikiewicz¹  Jan Gnus³  Wojciech Witkiewicz²  Krystyna Dąbrowska¹ 

[1] Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences;Regional Specialist Hospital in Wrocław, Research and Development Center;Medical Academy in Wroclaw

关键词: Helicobacter;    NGS;    Sequencing;    Pylori;    Stomach;    Next-generation sequencing;    Biopsy;    Specimens;   

DOI  :  10.7717/peerj.9099

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Helicobacter pylori is one of the major stomach microbiome components, promoting development of inflammation and gastric cancer in humans. H. pylori has a unique ability to transform into a coccoidal form which is difficult to detect by many diagnostic methods, such as urease activity detection, and even histopathological examination. Here we present a comparison of three methods for H. pylori identification: histological assessment (with eosin, hematoxylin, and Giemsa staining), polymerase chain reaction (PCR) detection of urease (ureA specific primers), and detection by 16S rRNA gene sequencing. The study employed biopsies from the antral part of the stomach (N = 40). All samples were assessed histologically which revealed H. pylori in eight patients. Bacterial DNA isolated from the bioptates was used as a template for PCR reaction and 16S rRNA gene sequencing that revealed H. pylori in 13 and in 20 patients, respectively. Thus, 16S rRNA gene sequencing was the most sensitive method for detection of H. pylori in stomach biopsy samples.

【 授权许可】

CC BY