Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali

PeerJ

article

Chuang Mei¹  Jie Yang¹  Peng Yan²  Ning Li²  Kai Ma²  Aisajan Mamat²  Liqun Han²  Qinglong Dong¹  Ke Mao¹  Fengwang Ma¹  Jixun Wang² 

[1] State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University;Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology ,(Xinjiang), Ministry of Agriculture

关键词: Malus sieversii;    Agrilus mali;    Gene discovery;    Full-length transcriptome;    Insect resistance;   

DOI  :  10.7717/peerj.8992

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Malus sieversii is the wild progenitor for many cultivars of domesticated apple and an important germplasm resource for breeding. However, this valuable species faces a significant threat in the areas north of the Tianshan Mountains in China, by the invasion of Agrilus mali, a destructive pest of apple trees belonging to the family Buprestidae. Our preliminary study has has shown that there may be resistance to this insect in M. sieversii plants in the field, but the corresponding molecular mechanisms remain unclear. In this study, we compared the response of insect-resistant and insect-susceptible plants of M. sieversii to insect feeding using full-length transcriptome and targeted metabolome. 112,103 non-chimeric full-length reads (FLNC) totaling 10.52 Gb of data were generating with Pacific Biosciences SingleMolecule, Real-Time (PacBio SMRT) sequencing. A total of 130.06 Gb data of long reads were acquired with an Illumina HiSeq. Function annotation indicated that the different expressed genes (DEGs) were mainly involved in signal transduction pathway of plant hormones and in the synthesis of compounds such as terpenes, quinones, flavonoids, and jasmonic acid. Through targeted metabolome analysis resistant strains showed higher levels of trans-cinnamic acid, caffeine and ferulic acid after pest infestation. This study helps to decipher the transcriptional changes and related signaling paths in M. sieversii after an insect feeding, which lays a foundation for further research on molecular mechanisms of insect resistance in apples.

【 授权许可】

CC BY