Changes of liver transcriptome profiles following oxidative stress in streptozotocin-induced diabetes in mice

PeerJ

article

Shuren Guo¹  Xiaohuan Mao²  Yunmeng Yan¹  Yan Zhang¹  Liang Ming¹ 

[1] Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University;Department of Clinical Laboratory, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University

关键词: Oxidative-stress;    STZ;    Differentially expressed RNAs;    Metabolism;    Liver;   

DOI  :  10.7717/peerj.8983

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundOxidative-stress (OS) was causal in the development of cell dysfunction and insulin resistance. Streptozotocin (STZ) was an alkylation agent that increased reactive oxygen species (ROS) levels. Here we aimed to explore the oxidative-stress and related RNAs in the liver of STZ-induced diabetic mice.MethodsRNA-sequencing was performed using liver tissues from STZ induced diabetic mice and controls. Pathway and Gene Ontology (GO) analyses were utilized to annotate the target genes. The differentially expressed RNAs involved in the peroxisome pathway were validated by qRT-PCR. The glucose metabolite and OS markers were measured in the normal control (NC) and STZ-induced diabetic mellitus (DM) group.ResultsThe levels of serum Fasting insulin, HbA1c, Malondialdehyde (MDA) and 8-iso-prostaglandin F2α (8-iso-PGF2α) were significant higher in DM groups than NC group, while SOD activity decreased significantly in DM groups. We found 416 lncRNAs and 910 mRNAs were differentially expressed in the STZ-induced diabetic mice compared to the control group. OS associated RNAs were differentially expressed in the liver of STZ-induced diabetic mice.ConclusionThis study confirmed that the OS was increased in the STZ-induced DM mice as evidenced by the increase of lipid peroxidation product MDA and 8-iso-PGF2α, identified aberrantly expressed lncRNAs and mRNAs in STZ-induced diabetic mice.

【 授权许可】

CC BY