Identification of DEGs and transcription factors involved in H. pylori -associated inflammation and their relevance with gastric cancer

PeerJ

article

Honghao Yin¹  Aining Chu¹  Songyi Liu¹  Yuan Yuan¹  Yuehua Gong¹ 

[1]Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Hospital of China Medical University

[2]Key Laboratory of Cancer Etiology and Prevention in Liaoning Education Department, the First Hospital of China Medical University

[3]Key Laboratory of GI Cancer Etiology and Prevention in Liaoning Province, the First Hospital of China Medical University

关键词: H. pylori;    Inflammation;    Gastric cancer;    DEGs;    Transcription factor;    Regulatory network;   

DOI  :  10.7717/peerj.9223

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundPrevious studies have indicated that chronic inflammation linked to H. pylori infection is the leading causes for gastric cancer (GC). However, the exact mechanism is not entirely clear until now.PurposeTo identify the key molecules and TFs involved in H. pylori infection and to provide new insights into H. pylori-associated carcinogenesis and lay the groundwork for the prevention of GC.ResultsGO and KEGG analysis revealed that the DEGs of Hp+-NAG were mainly associated with the immune response, chemokine activity, extracellular region and rheumatoid arthritis pathway. The DEGs of Hp+-AG-IM were related to the apical plasma membrane, intestinal cholesterol absorption, transporter activity and fat digestion and absorption pathway. In Hp+-NAG network, the expression of TNF, CXCL8, MMP9, CXCL9, CXCL1, CCL20, CTLA4, CXCL2, C3, SAA1 and FOXP3, JUN had statistical significance between normal and cancer in TCGA database. In Hp+-AG-IM network the expression of APOA4, GCG, CYP3A4, XPNPEP2 and FOXP3, JUN were statistically different in the comparison of normal and cancer in TCGA database. FOXP3 were negatively associated with overall survival, and the association for JUN was positive.ConclusionThe current study identified key DEGs and their transcriptional regulatory networks involved in H. pylori-associated NAG, AG-IM and GC and found that patients with higher expressed FOXP3 or lower expressed JUN had shorter overall survival time. Our study provided new directions for inflammation-associated oncogenic transformation involved in H. pylori infection.

【 授权许可】

CC BY