Chlorine-based DUWL disinfectant leads to a different microbial composition of water derived biofilms compared to H 2 O 2 -based chemical disinfectants in vitro

PeerJ

article

Charifa Zemouri¹  Alexa M.G.A. Laheij¹  Catherine M.C. Volgenant¹  Bernd W. Brandt¹  Wim Crielaard¹  Mark J. Buijs¹  Egija Zaura¹  Johannes J. de Soet¹ 

[1] Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam

关键词: Biofilm;    Dental unit waterlines;    Disinfection;    Microbiome;    Sequencing;    Water quality;   

DOI  :  10.7717/peerj.9503

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundBiofilm formation in dental unit waterlines (DUWL) may lead to health risks for dental staff and patients. Therefore, dental unit waterlines need to be disinfected, for instance by using chemical disinfectants. However, the application of chemical disinfectants may lead to the selection of specific microorganisms. Therefore, the aim of our study was to assess the microbial composition of water-derived biofilms, after a continuous exposure to maintenance doses of commercially available chemical disinfectants, in vitro.MethodsThe AAA-model was used to grow water derived biofilms. The biofilms were subjected to the maintenance dose of each disinfectant. To determine the microbial composition, the V4 hypervariable region of the 16S rRNA gene was sequenced. The sequences were clustered in operational taxonomic units (OTUs).ResultsThe bacterial composition of biofilms in all treatment groups differed significantly (PERMANOVA F = 4.441, p = 0.001). Pairwise comparisons revealed Anoxyl treated biofilms were significantly different from all groups (p = 0.0001). In the Anoxyl-treated biofilms, the relative abundance of Comamonadaceae and Sphingopyxis was high compared to the Dentosept, Green and Clean and Oxygenal groups.ConclusionWe concluded that exposure to low doses of the chlorine-based chemical disinfectant Anoxyl led to a substantially different composition of water derived biofilms compared to biofilms exposed to H2O2-based chemical disinfectants.

【 授权许可】

CC BY