Antibacterial activity and mechanism of sanguinarine against Providencia rettgeri in vitro

PeerJ

article

Qian Zhang¹  Yansi Lyu¹  Jingkai Huang¹  Xiaodong Zhang¹  Na Yu¹  Ziping Wen¹  Si Chen³ 

[1] Department of Dermatology, Shenzhen University General Hospital, Shenzhen University;College of Physics and Optoelectronic Engineering, Shenzhen University;Shenzhen University Health Science Center;Department of Dermatology, PLAGH Hainan Hospital Of PLA General Hospital;Department of Immunology, Shenzhen University School of Medicine

关键词: Sanguinarine;    Providencia rettgeri;    Antimicrobial;    Antibiofilm;   

DOI  :  10.7717/peerj.9543

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Sanguinarine (SAG), a benzophenanthridine alkaloid, occurs in Papaveraceas, Berberidaceae and Ranunculaceae families. Studies have found that SAG has antioxidant, anti-inflammatory, and antiproliferative activities in several malignancies and that it exhibits robust antibacterial activities. However, information reported on the action of SAG against Providencia rettgeri is limited in the literature. Therefore, the present study aimed to evaluate the antimicrobial and antibiofilm activities of SAG against P. rettgeri in vitro. Methods The agar dilution method was used to determine the minimum inhibitory concentration (MIC) of SAG against P. rettgeri. The intracellular ATP concentration, intracellular pH (pHin), and cell membrane integrity and potential were measured. Confocal laser scanning microscopy (CLSM), field emission scanning electron microscopy (FESEM), and crystal violet staining were used to measure the antibiofilm formation of SAG. Results The MIC of SAG against P. rettgeri was 7.8 μg/mL. SAG inhibited the growth of P. rettgeri and destroyed the integrity of P. rettgeri cell membrane, as reflected mainly through the decreases in the intracellular ATP concentration, pHin and cell membrane potential and significant changes in cellular morphology. The findings of CLSM, FESEM and crystal violet staining indicated that SAG exhibited strong inhibitory effects on the biofilm formation of P. rettgeri and led to the inactivity of biofilm-related P. rettgeri cells.

【 授权许可】

CC BY