Methylene blue inhibits nucleation and elongation of SOD1 amyloid fibrils

PeerJ

article

Greta Musteikyte¹  Mantas Ziaunys²  Vytautas Smirnovas² 

[1] Department of Chemistry, University of Cambridge;Life Sciences Center, Institute of Biotechnology, Vilnius University

关键词: Amyloid;    Protein misfolding;    Methylene blue;    Superoxide dismutase;    Protein aggregation;    Inhibition;    Nucleation;    Fibril elongation;   

DOI  :  10.7717/peerj.9719

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Protein aggregation into highly-structured amyloid fibrils is linked to several neurodegenerative diseases. Such fibril formation by superoxide dismutase I (SOD1) is considered to be related to amyotrophic lateral sclerosis, a late-onset and fatal disorder. Despite much effort and the discovery of numerous anti-amyloid compounds, no effective cure or treatment is currently available. Methylene blue (MB), a phenothiazine dye, has been shown to modulate the aggregation of multiple amyloidogenic proteins. In this work we show its ability to inhibit both the spontaneous amyloid aggregation of SOD1 as well as elongation of preformed fibrils.

【 授权许可】

CC BY