Identification of fertility-related genes for maize CMS-S via Bulked Segregant RNA-Seq

PeerJ

article

Xiner Qin¹  Wenliang Zhang¹  Xue Dong¹  Shike Tian¹  Panpan Zhang¹  Yanxin Zhao²  Yi Wang³  Jianbing Yan¹  Bing Yue¹ 

[1] National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University;Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences;Industrial Crops Research Institution, Heilongjiang Academy of Land Reclamation of Sciences

关键词: BSR-Seq;    Differentially expressed genes;    Fertility restoration;    CMS-S;    Maize;   

DOI  :  10.7717/peerj.10015

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Cytoplasmic male sterility (CMS) is extensively used in maize hybrid production, and identification of genes related to fertility restoration for CMS is important for hybrid breeding. The fertility restoration of S type CMS is governed by several loci with major and minor effects, while the mechanism of fertility restoration for CMS-S is still unknown. In this study, BSR-Seq was conducted with two backcrossing populations with the fertility restoration genes, Rf3 and Rf10, respectively. Genetic mapping via BSR-Seq verified the positions of the two loci. A total of 353 and 176 differentially expressed genes (DEGs) between the male fertility and male sterile pools were identified in the populations with Rf3 and Rf10, respectively. In total, 265 DEGs were co-expressed in the two populations, which were up-regulated in the fertile plants, and they might be related to male fertility involving in anther or pollen development. Moreover, 35 and seven DEGs were specifically up-regulated in the fertile plants of the population with Rf3 and Rf10, respectively. Function analysis of these DEGs revealed that jasmonic acid (JA) signal pathway might be involved in the Rf3 mediated fertility restoration for CMS-S, while the small ubiquitin-related modifier system could play a role in the fertility restoration of Rf10.

【 授权许可】

CC BY