Isolation and evolutionary analyses of porcine epidemic diarrhea virus in Asia

PeerJ

article

Zewen Liu¹  Ting Gao¹  Ling Zhao²  Dongwan Yoo³  Yongxiang Tian¹  Wan Liang¹  Danna Zhou¹  Chao Geng¹  Keli Yang¹  Zhengying Duan¹  Rui Guo¹  Wei Liu¹  Fangyan Yuan¹ 

[1] Key Laboratory of Prevention and Control Agents for Animal Bacteriosis ,(Ministry of Agriculture), Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences;Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University;Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign;College of Animal Sciences, Yangtze University

关键词: Porcine epidemic diarrhea virus;    Virus isolation;    Genetic evolutionary analysis;    Amino acid mutations;    Asia;   

DOI  :  10.7717/peerj.10114

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Porcine epidemic diarrhea virus (PEDV) is a leading cause of diarrhea in pigs worldwide. Virus isolation and genetic evolutionary analysis allow investigations into the prevalence of epidemic strains and provide data for the clinical diagnosis and vaccine development. In this study, we investigated the genetic characteristics of PEDV circulation in Asia through virus isolation and comparative genomics analysis. APEDV strain designated HB2018 was isolated from a pig in a farm experiencing a diarrhea outbreak. The complete genome sequence of HB2018 was 28,138 bp in length. Phylogenetic analysis of HB2018 and 207 PEDVs in Asia showed that most PEDV strains circulating in Asia after 2010 belong to genotype GII, particularly GII-a. The PEDV vaccine strain CV777 belonged to GI, and thus, unmatched genotypes between CV777 and GII-a variants might partially explain incomplete protection by the CV777-derived vaccine against PEDV variants in China. In addition, we found the S protein of variant strains contained numerous mutations compared to the S protein of CV777, and these mutations occurred in the N-terminal domain of the S protein. These mutations may influence the antigenicity, pathogenicity, and neutralization properties of the variant strains.

【 授权许可】

CC BY