Comparison of the diversity of cultured and total bacterial communities in marine sediment using culture-dependent and sequencing methods

PeerJ

article

Meng Wang¹  Samina Noor¹  Ran Huan¹  Congling Liu¹  JiaYi Li¹  Qingxin Shi¹  Yan-Jiao Zhang²  Cuiling Wu³  Hailun He¹ 

[1] School of Life Science, Central South University;Qingdao Agricultural University;Changzhi Medical College

关键词: Marine sediment;    Culturable microbial diversity;    Microbial diversity;    Media components;    Physiochemical parameters;   

DOI  :  10.7717/peerj.10060

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Despite recent great advances in microbial culture, most microbes have not yet been cultured, and the impact of medium composition on the isolation of microbes from natural systems has not been elucidated. To optimize media for culturing marine microbes, microbial communities in three sediment samples were described using high-throughput sequencing (HTS) and culture-dependent techniques. HTS revealed communities dominated by Gammaproteobacteria, and culture-based methods revealed communities dominated by Actinobacteria. Among the total operational taxonomic units (OTUs) from the HTS dataset, 6% were recovered in the culture collection. Four potentially novel bacterial strains belonging to Oceaniovalibus, Psychrobacter and Salegentibacter were isolated. The combination of media cultured more taxa than any single medium. Nutrient-rich and single-carbon/nitrogen-source media supported the growth of relatively few taxa, and the quality of nitrogen strongly influenced the types of bacteria isolated.

【 授权许可】

CC BY