DNA barcoding for identification of anuran species in the central region of South America

PeerJ

article

Ricardo Koroiva¹  Luís Reginaldo Ribeiro Rodrigues²  Diego José Santana³ 

[1] Departamento de Sistemática e Ecologia, Universidade Federal da Paraíba;Instituto de Ciências da Educação, Universidade Federal do Oeste do Pará;Instituto de Biociências, Universidade Federal de Mato Grosso do Sul

关键词: Anura;    Frog;    Mato Grosso do Sul;    DNA Barcode;    COI;    Brazil;   

DOI  :  10.7717/peerj.10189

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The use of COI barcodes for specimen identification and species discovery has been a useful molecular approach for the study of Anura. Here, we establish a comprehensive amphibian barcode reference database in a central area of South America, in particular for specimens collected in Mato Grosso do Sul state (Brazil), and to evaluate the applicability of the COI gene for species-level identification. Both distance- and tree-based methods were applied for assessing species boundaries and the accuracy of specimen identification was evaluated. A total of 204 mitochondrial COI barcode sequences were evaluated from 22 genera and 59 species (19 newly barcoded species). Our results indicate that morphological and molecular identifications converge for most species, however, some species may present cryptic species due to high intraspecific variation, and there is a high efficiency of specimen identification. Thus, we show that COI sequencing can be used to identify anuran species present in this region.

【 授权许可】

CC BY