Effects of different drying methods on smears of canine blood and effusion fluid

PeerJ

article

Fiamma G. De Witte¹  Aimee Hebrard²  Carolyn N. Grimes³  Kristin Owens⁴  Deanna M. Schaefer²  Xiaojuan Zhu²  Michael M. Fry² 

[1] BluePearl Veterinary Hospital;Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee;Ethos Diagnostics Science;Antech Diagnostics

关键词: Canine;    Smears;    Slides;    Blood;    Effusion;    Drying methods;    Hairdryer;   

DOI  :  10.7717/peerj.10092

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundGlass slide preparations from a variety of specimens (blood, masses, effusions) are commonly made as part of the diagnostic work-up, however the effects of various drying methods in veterinary practice and diagnostic laboratory settings is not clear.ObjectiveCompare the effects of four drying methods on results of microscopic examination of canine blood smears and direct smears of pleural or peritoneal effusion fluid.MethodsTwelve canine blood samples (6 from healthy dogs, 6 from sick dogs) and 6 canine peritoneal or pleural effusion samples. Four smears were prepared from each of the 18 samples and dried using the following methods: air-dry, hair dryer with or without heat, and heat block at 58 °C. Observers, blinded to the drying method, independently reviewed the slides microscopically, using a scoring system to evaluate cell morphology and (for blood smears) echinocyte numbers; scoring results were analyzed statistically.ResultsFor blood smears, several comparisons showed more adverse effects on morphology using the heat block method than for one or more other drying methods. For effusion fluid smears, RBCs dried with the heat block or air-dry methods had more poorly preserved morphology than RBCs dried by the hair dryer method without heat.Conclusions and clinical relevanceThe results (1) indicate that different drying methods had a significant effect, (2) support using a hair dryer without heat for both blood smears and effusion fluid smears, and (3) discourage using a 58 °C heat block.

【 授权许可】

CC BY