Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies

PeerJ

article

Carol Haddoub¹  Mohamad Rima²  Sandrine Heurtebise³  Myriam Lawand³  Dania Jundi⁴  Riyad Sadek⁶  Sebastian Amigorena³  Ziad Fajloun⁴  Marc C. Karam¹ 

[1]Department of Biology, University of Balamand

[2]Institut de Génétique et de Biologie Moléculaire et Cellulaire ,(IGBMC), INSERM, CNRS, University of Strasbourg

[3]Institut Curie, INSERM U932, PSL Research University

[4]LAB3B, Doctoral School for Sciences and Technology, Azm Centre for Research in Biotechnology, Lebanese University

[5]Department of Neuroscience, Institute of Biology Paris-Seine ,(IBPS), INSERM, CNRS, Université Sorbonne-Nouvelle

[6]Department of Biology, American University of Beirut

[7]Faculty of Sciences 3, Michel Slayman Tripoli Campus, Lebanese University

关键词: Montivipera bornmuelleri;    B16 cells;    MCA cells;    Anti-cancer activity;    In vivo;   

DOI  :  10.7717/peerj.9909

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundMontivipera bornmuelleri’s venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCA-induced murine fibrosarcoma cell lines to M. bornmuelleri’s venom and its effect on tumor growth in vivo. Methods The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleri’s venom in vitro which, however, does not translate to an anticancer action in vivo.

【 授权许可】

CC BY