Cathepsin D in prawn reproductive system: its localization and function in actin degradation

PeerJ

article

Chompoonut Sukonset¹  Piyaporn Surinlert²  Orawan Thongsum¹  Atthaboon Watthammawut³  Monsicha Somrit¹  Jirasuda Nakeim⁴  Wattana Weerachatyanukul¹  Somluk Asuvapongpatana¹ 

[1] Department of Anatomy, Faculty of Science, Mahidol University;Chulabhon International College of Medicine, Thammasat University;Department of Anatomy, Faculty of Medicine, Srinakharinwirot University;Department of Anatomy, Faculty of Allied Health Science, Buraphar University

关键词: CAT-D;    Testis;    Sperm;    Spermatogenesis;    Actin meshwork;   

DOI  :  10.7717/peerj.10218

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Cathepsin D (CAT-D) is a well-known aspartic protease that serves a function as house-keeping lysosomal enzyme in all somatic cells. Its existence in reproductive tissues is highly variable, even in the somatic derived epithelial cells of reproductive tract. In Macrobrachium rosenbergii, existence of MrCAT-D and its translational product was detected in both somatic cells (Sertoli-like supporting cells) and developing spermatogenic cells as well as along accessory spermatic ducts. Specifically, MrCAT-D was localized onto the sperm surface rather than within the acrosomal matrix, as evident by similar staining pattern of anti-CAT-D on live and aldehyde fixed sperm. MrCAT-D in testicular extracts and sperm isolates showed active enzyme activities towards its specific fluorogenic substrate (MCA-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys (Dnp)-D-Arg-NH2). MrCAT-D also exerted its function towards hydrolyzing filamentous actin, the meshwork of which is shown to be localized at the junction between germ cells and supporting cells and spermatogonia in M. rosenbergii testicular epithelium. Together, we have localized MrCAT-D transcript and its translational product in both supporting and germ cells of testis and claimed its enzymatic function towards actin degradation, which may be related to sperm release from the epithelial cell interaction.

【 授权许可】

CC BY