A diverse global fungal library for drug discovery

PeerJ

article

Guodong Niu¹  Thirunavukkarasu Annamalai²  Xiaohong Wang¹  Sheng Li³  Stephen Munga⁴  Guomin Niu⁵  Yuk-Ching Tse-Dinh²  Jun Li¹ 

[1] Department of Biological Sciences, Florida International University;Department of Chemistry and Biochemistry, Florida International University;School of Public Health, City University of New York;Center for Global Health Research, Kenya Medical Research Institute;Department of Hematology, Southern Medical University Affiliated Nanhai Hospital;Biomolecular Sciences Institute, Florida International University

关键词: Fungus;    Secondary metabolites;    Small molecule;    Natural product;    Drugs;    Anti-malaria;    Antibiotics;    Anti-leukemia;   

DOI  :  10.7717/peerj.10392

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundSecondary fungal metabolites are important sources for new drugs against infectious diseases and cancers.MethodsTo obtain a library with enough diversity, we collected about 2,395 soil samples and 2,324 plant samples from 36 regions in Africa, Asia, and North America. The collection areas covered various climate zones in the world. We examined the usability of the global fungal extract library (GFEL) against parasitic malaria transmission, Gram-positive and negative bacterial pathogens, and leukemia cells.ResultsNearly ten thousand fungal strains were isolated. Sequences of nuclear ribosomal internal transcribed spacer (ITS) from 40 randomly selected strains showed that over 80% were unique. Screening GFEL, we found that the fungal extract from Penicillium thomii was able to block Plasmodium falciparum transmission to Anopheles gambiae, and the fungal extract from Tolypocladium album was able to kill myelogenous leukemia cell line K562. We also identified a set of candidate fungal extracts against bacterial pathogens.

【 授权许可】

CC BY