Tracking the dynamics of individual gut microbiome of sea cucumber Apostichopus japonicus during gut regeneration

PeerJ

article

Yohei Yamazaki¹  Yuichi Sakai²  Juanwen Yu¹  Sayaka Mino¹  Tomoo Sawabe¹ 

[1] Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University;Hakodate Fisheries Research, Hokkaido Research Organization

关键词: Gut microbiome;    Gut regeneration;    Sea cucumber;    Apostichopus japonicus;   

DOI  :  10.7717/peerj.10260

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Sea cucumbers possess the remarkable capacity to regenerate their body parts or organs. Regeneration of host organs and/or body parts involves reconstruction of the host associated microbiota, however, the dynamics and contribution of microbiota to the regeneration process are largely unknown due to a lack of experimental models. To track the dynamics of individual gut microbiomes during gut regeneration, both caged mariculture and laboratory isolator systems of sea cucumbers (Apostichopus japonicus) were developed and longitudinal meta16S analyses were performed. Under natural environmental conditions in the caged mariculture system, both bacterial and eukaryotic communities in sea cucumbers’ guts appeared to be reconstructed within 4 months after evisceration. Using the laboratory isolator, which can trace daily dynamics, we found that fecal microbiota collected before evisceration were clearly different from those collected after evisceration. We also identified eight key bacteria, belonging to Alteromonadaceae, Rhodobacteraceae, Oceanospirillaceae and family-unassigned Gammaproteobacteria, suggesting that these bacteria might interact with the host during the gut regeneration process. Six of the eight key bacteria were isolated for further bioassay using the isolator developed in this study to test whether these isolates affect gut regeneration.

【 授权许可】

CC BY