Salivary microbial changes during the first 6 months of orthodontic treatment

PeerJ

article

Mei Zhao¹  Min Liu¹  Wei Chen¹  Haiping Zhang²  Yuxing Bai²  Wen Ren¹ 

[1] Department of Preventive Dentistry, School of Stomatology, Capital Medical University;Department of Orthodontics, School of Stomatology, Capital Medical University

关键词: Orthodontic treatment;    Microbiome;    16S rRNA gene high-throughput sequencing;   

DOI  :  10.7717/peerj.10446

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundOrthodontic treatment is widely used to treat malocclusion. However, the influence of treatment on the oral microbiome remains unclear. In this study, we investigated salivary microbial changes in patients undergoing orthodontic treatment.MethodsIn total, 19 orthodontic patients participated in this study. Saliva samples were collected at the following three timepoints: before (T0) and 3 months (T1) and 6 months (T2) after the placement of orthodontic appliances. High-throughput sequencing was performed based on the 16S rRNA gene V4 region.ResultsThe phyla of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Fusobacteria were predominant. Observed Species, Chao1 and ACE, which represent α diversity, were significantly decreased at T1 and subsequently increased at T2. In addition, the β diversity at T1 based on the Bray-Curtis distances differed from T0 and T2. The relative abundances of Prevotella, Porphyromonas and Peptostreptococcus were decreased with treatment, whereas those of Capnocytophaga and Neisseria exhibited the opposite results. In total, 385 of 410 operational taxonomic units were shared at T0, T1 and T2. The co-occurrence networks with hub nodes at T1 were the most complex.ConclusionOrthodontic treatment temporarily affected the saliva microbial community. This dynamic alteration in species did not induce deterioration in oral health. Oral hygiene instructions were necessary and should be emphasized during each visit. Further studies with longer observation periods and more participants are required.

【 授权许可】

CC BY