Dnajb8 , a target gene of SOX30, is dispensable for male fertility in mice

PeerJ

article

Fengsong Wang¹  Shuai Kong¹  Xuechun Hu²  Xin Li³  Bo Xu⁴  Qiuling Yue⁵  Kaiqiang Fu⁶  Lan Ye³  Shun Bai⁴ 

[1] School of Life Science, Anhui Medical University;Department of Urology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China;State Key Laboratory of Reproductive Medicine, Nanjing Medical University;Reproductive and Genetic Hospital, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China;Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School;College of Veterinary Medicine, Qingdao Agricultural University

关键词: Dnajb8;    Male fertility;    Spermatogenesis;   

DOI  :  10.7717/peerj.10582

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes.MethodsWe used the CRISPR/Cas9 system to generate Dnajb8 mutant mice and analyze the phenotype of the Dnajb8 mutants.ResultsAlthoughDnajb8 is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups.

【 授权许可】

CC BY