Microbial community structure in aquifers associated with arsenic: analysis of 16S rRNA and arsenite oxidase genes

PeerJ

article

Prinpida Sonthiphand¹  Pasunun Rattanaroongrot¹  Kasarnchon Mek-yong¹  Kanthida Kusonmano²  Chalida Rangsiwutisak²  Pichahpuk Uthaipaisanwong²  Srilert Chotpantarat⁴  Teerasit Termsaithong⁷ 

[1] Department of Biology, Faculty of Science, Mahidol University;Bioinformatics and Systems Biology Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi;Systems Biology and Bioinformatics Research Laboratory, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi;Department of Geology, Faculty of Science, Chulalongkorn University;Research Program on Controls of Hazardous Contaminants in Raw Water Resources for Water Scarcity Resilience, Center of Excellence on Hazardous Substance Management ,(HSM), Chulalongkorn University;Research Unit of Green Mining ,(GMM), Chulalongkorn University;Learning Institute, King Mongkut’s University of Technology Thonburi;Theoretical and Computational Science Center ,(TaCS), King Mongkut’s University of Technology Thonburi

关键词: Microbiome;    Deep groundwater;    Shallow groundwater;    AioA gene;    Arsenite-oxidizing bacteria;    Arsenic;    Arsenite oxidase;   

DOI  :  10.7717/peerj.10653

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The microbiomes of deep and shallow aquifers located in an agricultural area, impacted by an old tin mine, were explored to understand spatial variation in microbial community structures and identify environmental factors influencing microbial distribution patterns through the analysis of 16S rRNA and aioA genes. Although Proteobacteria, Cyanobacteria, Actinobacteria, Patescibacteria, Bacteroidetes, and Epsilonbacteraeota were widespread across the analyzed aquifers, the dominant taxa found in each aquifer were unique. The co-dominance of Burkholderiaceae and Gallionellaceae potentially controlled arsenic immobilization in the aquifers. Analysis of the aioA gene suggested that arsenite-oxidizing bacteria phylogenetically associated with Alpha-, Beta-, and Gamma proteobacteria were present at low abundance (0.85 to 37.13%) and were more prevalent in shallow aquifers and surface water. The concentrations of dissolved oxygen and total phosphorus significantly governed the microbiomes analyzed in this study, while the combination of NO3--N concentration and oxidation-reduction potential significantly influenced the diversity and abundance of arsenite-oxidizing bacteria in the aquifers. The knowledge of microbial community structures and functions in relation to deep and shallow aquifers is required for further development of sustainable aquifer management.

【 授权许可】

CC BY