Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ

article

Muniba Tariq¹  Hafiz Muhammad Tahir¹  Samima Asad Butt¹  Shaukat Ali¹  Asma Bashir Ahmad¹  Chand Raza¹  Muhammad Summer¹  Ali Hassan¹  Junaid Nadeem¹ 

[1] Department of Zoology, Government College University Lahore

关键词: Sericin;    Fibroin;    Silk;    Diabetes;    Aloe vera;    Skin wound;   

DOI  :  10.7717/peerj.10232

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

【 授权许可】

CC BY