Diversity of microbes colonizing forages of varying lignocellulose properties in the sheep rumen

PeerJ

article

Mohammad Farhad Vahidi¹  Javad Gharechahi²  Mehrdad Behmanesh¹  Xue-Zhi Ding³  Jian-Lin Han⁴  Ghasem Hosseini Salekdeh⁶ 

[1] Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University;Human Genetics Research Center, Baqiyatallah University of Medical Sciences;Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences;Livestock Genetics Program, International Livestock Research Institute;CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences ,(CAAS), Beijing, China, Institute of Animal Science;Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research

关键词: Sheep;    Rumen incubation;    Biomass degradation;    Microbiome;    16S rRNA gene sequencing;   

DOI  :  10.7717/peerj.10463

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe rumen microbiota contributes strongly to the degradation of ingested plant materials. There is limited knowledge about the diversity of taxa involved in the breakdown of lignocellulosic biomasses with varying chemical compositions in the rumen.MethodWe aimed to assess how and to what extent the physicochemical properties of forages influence the colonization and digestion by rumen microbiota. This was achieved by placing nylon bags filled with candidate materials in the rumen of fistulated sheep for a period of up to 96 h, followed by measuring forage’s chemical characteristics and community structure of biofilm-embedded microbiota.ResultsRumen degradation for all forages appeared to have occurred mainly during the first 24 h of their incubation, which significantly slowed down after 48 h of rumen incubation, depending on their chemical properties. Random Forest analysis predicted the predominant role of Treponema and Butyrivibrio in shaping microbial diversity attached to the forages during the course of rumen incubation. Exploring community structure and composition of fiber-attached microbiota revealed significant differential colonization rates of forages depending on their contents for NDF and cellulose. The correlation analysis highlighted the significant contribution of Lachnospiraceae and Veillonellaceae to fiber degradation in the sheep rumen.ConclusionOur findings suggested that forage cellulose components are critical in shaping the pattern of microbial colonization and thus their final digestibility in the rumen.

【 授权许可】

CC BY