Metabolic impact of weight variations in Icelandic horses

PeerJ

article

Julien Delarocque¹  Florian Frers¹  Korinna Huber²  Klaus Jung³  Karsten Feige¹  Tobias Warnken¹ 

[1] Clinic for Horses, University of Veterinary Medicine Hannover, Foundation;Institute of Animal Science, Faculty of Agricultural Sciences, Universität Hohenheim;Institute for Animal Breeding and Genetics

关键词: Equine metabolic syndrome;    Insulin dysregulation;    Oral glucose test;    Obesity;    Metabolomics;    Pathway analysis;   

DOI  :  10.7717/peerj.10764

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundInsulin dysregulation (ID) is an equine endocrine disorder, which is often accompanied by obesity and various metabolic perturbations. The relationship between weight variations and fluctuations of the insulin response to oral glucose tests (OGT) as well as the metabolic impact of ID have been described previously. The present study seeks to characterize the concomitant metabolic impact of variations in the insulin response and bodyweight during repeated OGTs using a metabolomics approach.MethodsNineteen Icelandic horses were subjected to five OGTs over one year and their bodyweight, insulin and metabolic response were monitored. Analysis of metabolite concentrations depending on time (during the OGT), relative bodyweight (rWeight; defined as the bodyweight at one OGT divided by the mean bodyweight across all OGTs) and relative insulin response (rAUCins; defined accordingly from the area under the insulin curve during OGT) was performed using linear models. Additionally, the pathways significantly associated with time, rWeight and rAUCins were identified by rotation set testing.ResultsThe results suggested that weight gain and worsening of ID activate distinct metabolic pathways. The metabolic profile associated with weight gain indicated an increased activation of arginase, while the pathways associated with time and rAUCins were consistent with the expected effect of glucose and insulin, respectively. Overall, more metabolites were significantly associated with rWeight than with rAUCins.

【 授权许可】

CC BY