Genome-wide identification and expression analysis of ADP-ribosylation factors associated with biotic and abiotic stress in wheat ( Triticum aestivum L.)

PeerJ

article

Yaqian Li¹  Jinghan Song¹  Guang Zhu¹  Zehao Hou²  Lin Wang²  Xiaoxue Wu²  Zhengwu Fang²  Yike Liu¹  Chunbao Gao¹ 

[1] Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences/Hubei Engineering and Technology Research Center of Wheat/Wheat Disease Biology Research Station for Central China;Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University

关键词: Wheat;    TaARF;    Genome-wide;    Phylogenetic analysis;    Gene expression;   

DOI  :  10.7717/peerj.10963

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The ARF gene family plays important roles in intracellular transport in eukaryotes and is involved in conferring tolerance to biotic and abiotic stresses in plants. To explore the role of these genes in the development of wheat (Triticum aestivum L.), 74 wheat ARF genes (TaARFs; including 18 alternate transcripts) were identified and clustered into seven sub-groups. Phylogenetic analysis revealed that TaARFA1 sub-group genes were strongly conserved. Numerous cis-elements functionally associated with the stress response and hormones were identified in the TaARFA1 sub-group, implying that these TaARFs are induced in response to abiotic and biotic stresses in wheat. According to available transcriptome data and qRT-PCR analysis, the TaARFA1 genes displayed tissue-specific expression patterns and were regulated by biotic stress (powdery mildew and stripe rust) and abiotic stress (cold, heat, ABA, drought and NaCl). Protein interaction network analysis further indicated that TaARFA1 proteins may interact with protein phosphatase 2C (PP2C), which is a key protein in the ABA signaling pathway. This comprehensive analysis will be useful for further functional characterization of TaARF genes and the development of high-quality wheat varieties.

【 授权许可】

CC BY