Detection of sexually transmitted pathogens and co-infection with human papillomavirus in women residing in rural Eastern Cape, South Africa

PeerJ

article

Ongeziwe Taku¹  Adrian Brink³  Tracy L. Meiring¹  Keletso Phohlo¹  Charles B. Businge⁴  Zizipho Z.A. Mbulawa¹  Anna-Lise Williamson¹ 

[1] Department of Pathology, Faculty of health sciences, University of Cape Town;Institute of Infectious Disease and Molecular Medicine, University of Cape Town;Division of Medical Microbiology, Department of Pathology, Faculty of Health Sciences, University of Cape Town;Department of Obstetrics and Gynaecology, Nelson Mandela Academic Hospital;Department of Obstetrics and Gynaecology, Faculty of Health Sciences, Walter Sisulu University;SAMRC Gynaecological Cancer Research Centre, University of Cape Town;Department of Laboratory Medicine and Pathology, Walter Sisulu University;National Health Laboratory Service, Nelson Mandela Academic Hospital

关键词: HPV;    Sexually transmitted pathogens;    Treponema pallidum;    Trichomonas Vaginalis;    Mycoplasma genitalium;    Mycoplasma hominis;    Ureaplasma;   

DOI  :  10.7717/peerj.10793

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundSouth African women of reproductive age have a high burden of sexually transmitted infections (STIs), including human papillomavirus (HPV) infection. However, there is limited information on the prevalence of sexually transmitted pathogens in women from rural Eastern Cape Province, South Africa. The study aims at determining the prevalence of sexually transmitted pathogens and co-infection with high-risk (HR) HPV among women from rural Eastern Cape Province, South Africa.MethodsA total of 205 cervical specimens were collected from women aged ≥ 30 years from a rural community-based clinic. The samples were tested for a panel of pathogenic STIs [Chlamydia trachomatis (serovars A-K & L1-L3), Haemophilus ducreyi, Herpes Simplex Virus (Types 1 & 2), Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis (TV), and pathobionts [Mycoplasma genitalium (MG), Mycoplasma hominis (MH) and Ureaplasma spp. (UP)] using a multiplex PCR STD direct flow chip assay through a manual Hybrispot platform (Master Diagnostica, Granada, Spain). HR-HPV detection was performed by Hybrid Capture-2 assay.ResultsHigh-risk HPV prevalence was 32.2% (66/205) and HIV-1 prevalence was 38.5% (79/205). The overall prevalence of six pathogenic STIs was 22.9% (47/205), with TV having the highest prevalence (15.6%; 32/205). UP (70.2%, 144/205) and MH (36.6%, 75/205) were the most frequently detected pathobionts. Co-infection with ≥ 2 pathogens pathobionts was observed among 52.7% (108/205) participants. Of the six pathogenic STIs, three participants had more than one STI (1.46%) with the presence of MH and UP. HSV-2 (OR: 4.17, CI [1.184–14.690]) and HIV infection (OR: 2.11, CI [1.145–3.873]) were independent STIs associated with HR-HPV infection.ConclusionsThe high prevalence of pathogenic STIs underscores the need to improve syndromic management policy by implementing effective strategies of prevention, screening tests, and management. HSV-2 and HIV positive remain strongly associated with HR-HPV infection.

【 授权许可】

CC BY