Proteomic analysis reveals key proteins involved in ethylene-induced adventitious root development in cucumber ( Cucumis sativus L.)

PeerJ

article

Jian Lyu¹  Yue Wu¹  Xin Jin²  Zhongqi Tang¹  Weibiao Liao¹  Mohammed Mujitaba Dawuda¹  Linli Hu¹  Jianming Xie¹  Jihua Yu¹  Alejandro Calderón-Urrea⁵ 

[1] College of Horticulture, Gansu Agricultural University;College of Agriculture and Forestry Science, Linyi University;Department of Horticulture, University for Development Studies;Gansu Provincial Key Laboratory of Arid Land Crop Science, Gansu Agricultural University;Department of Biology, College of Science and Mathematics, California State University;College of Plant Protection, Gansu Agricultural University

关键词: Ethylene;    Adventitious root;    Proteomics;    Ethylene synthesis;    Photosynthetic carbon assimilation;   

DOI  :  10.7717/peerj.10887

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The mechanisms involved in adventitious root formation reflect the adaptability of plants to the environment. Moreover, the rooting process is regulated by endogenous hormone signals. Ethylene, a signaling hormone molecule, has been shown to play an essential role in the process of root development. In the present study, in order to explore the relationship between the ethylene-induced adventitious rooting process and photosynthesis and energy metabolism, the iTRAQ technique and proteomic analysis were employed to ascertain the expression of different proteins that occur during adventitious rooting in cucumber (Cucumis sativus L.) seedlings. Out of the 5,014 differentially expressed proteins (DEPs), there were 115 identified DEPs, among which 24 were considered related to adventitious root development. Most of the identified proteins were related to carbon and energy metabolism, photosynthesis, transcription, translation and amino acid metabolism. Subsequently, we focused on S-adenosylmethionine synthase (SAMS) and ATP synthase subunit a (AtpA). Our findings suggest that the key enzyme, SAMS, upstream of ethylene synthesis, is directly involved in adventitious root development in cucumber. Meanwhile, AtpA may be positively correlated with photosynthetic capacity during adventitious root development. Moreover, endogenous ethylene synthesis, photosynthesis, carbon assimilation capacity, and energy material metabolism were enhanced by exogenous ethylene application during adventitious rooting. In conclusion, endogenous ethylene synthesis can be improved by exogenous ethylene additions to stimulate the induction and formation of adventitious roots. Moreover, photosynthesis and starch degradation were enhanced by ethylene treatment to provide more energy and carbon sources for the rooting process.

【 授权许可】

CC BY