Identification and comparison of circular RNAs in preeclampsia

PeerJ

article

Zepeng Ping¹  Ling Ai¹  Huaxiang Shen¹  Xing Zhang²  Huling Jiang¹  Ye Song³ 

[1] Department of Obstetrics, Maternity and Child Health Care Affiliated Hospital, Jiaxing University;School of Biology & Basic Medical Science, Soochow University;Department of Obstetrics, Suzhou Municipal Hospital

关键词: Preeclampsia;    circRNA;    Expression pattern;    miRNA;    ceRNA;   

DOI  :  10.7717/peerj.11299

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundPreeclampsia (PE) is a pregnancy-specific syndrome, belongs to the gestational hypertension diseases category and is considered among the causes of maternal and perinatal mortality and morbidity. However, the pathogenesis of PE is still vague.MethodsIn the present study, the circular RNA (circRNA) expression patterns of normal pregnant women and PE patients were investigated using whole RNA sequencing.ResultsA total of 151 differential expressed circRNAs were identified including 121 upregulated and 30 downregulated ones. Functional and pathway enrichment analysis was conducted on the differentially expressed circRNAs using Gene Ontology and KEGG databases. The results of this analysis indicated that several crucial biological processes and pathways were enriched in PE patients. circRNA–microRNA (miRNA) interaction analysis indicated that the reported differentially expresse circRNAs may be associated with some regulatory functions through miRNAs in PE patients. Two ceRNAs networks were constructed according to the targeting relationship between circRNAs/miRNAs and miRNAs/mRNAs. One sub-network contained one upregulated circRNA, four downregulated miRNAs and five upregulated mRNAs, and another sub-network contained 10 downregulated circRNAs, 21 upregulated miRNAs and 15 downregulated mRNAs.ConclusionCircRNA expression patterns have been investigated and this analysis revealed their potential regulatory mechanisms in PE patients. We constructed the ceRNAs (competing endogenous RNA) to reveal the potential molecular roles of dysregulated circRNAs in the PE patients using RNA sequencing data. circRNA_13301 was the only one upregulated circRNA in ceRNA being targeted by four miRNAs.

【 授权许可】

CC BY