Increased microRNA-30a levels in bronchoalveolar lavage fluid as a diagnostic biomarker for lung cancer

PeerJ

article

Wen-Jun Zhu¹  Bo-Jiang Chen¹  Ying-Ying Zhu¹  Ling Sun¹  Yu-Chen Zhang¹  Huan Liu³  Feng-Ming Luo¹ 

[1]Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University

[2]Laboratory of Pulmonary Immunology and Inflammation, Frontiers Science Center for Disease-related Molecular Network, Sichuan University

[3]Department of General Medicine, University-Town Hospital of Chongqing Medical University

关键词: miRNA-30a;    Lung cancer;    Bronchoalveolar lavage fluid (BALF);    Tumor marker;    Early diagnosis;    Biomarker;   

DOI  :  10.7717/peerj.11528

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundMicroRNA-30a (miRNA-30a) levels have been shown to increase in the plasma of lung cancer patients. Herein, we evaluated the miRNA-30a levels in the bronchoalveolar lavage fluid (BALF) of lung cancer patients as a potential biomarker for lung cancer diagnosis.MethodsBALF miRNA-30a expression of 174 subjects was quantified using quantitative real-time reverse transcription-polymerase chain reaction and compared between lung cancer patients and control patients with benign lung diseases. Moreover, its diagnostic value was evaluated by performing receiver operating characteristic (ROC) curve analysis.ResultsThe relative BALF miRNA-30a expression was significantly higher in the lung cancer patients than in the controls (0.74 ±  0.55 versus 0.07 ±  0.48, respectively, p < 0.001) as well as in lung cancer patients with stage I–IIA disease than in those with stage IIB–IV disease (0.98 ±  0.64 versus 0.66 ±  0.54, respectively, p < 0.05). Additionally, miRNA-30a distinguished benign lung diseases from lung cancers, with an area under the ROC curve (AUC) of 0.822. ROC analysis also revealed an AUC of 0.875 for the Youden index-based optimal cut-off points for stage I–IIA adenocarcinoma. Thus, increased miRNA-30a levels in BALF may be a useful biomarker for non-small-cell lung cancer diagnosis.

【 授权许可】

CC BY