Transcriptome analysis associated with polysaccharide synthesis and their antioxidant activity in Cyclocarya paliurus leaves of different developmental stages

PeerJ

article

Weida Lin¹  Huanwei Chen³  Jianmei Wang⁴  Yongli Zheng⁵  Qiuwei Lu¹  Ziping Zhu¹  Na Li¹  Zexin Jin¹  Junmin Li¹  Hongfei Lu² 

[1] Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University;College of Life Science and Medicine, Zhejiang Sci-Tech University;Forest Research Institute of Longquan City;Zhejiang Yuanyang Agriculture Development Co. Ltd;Zhejiang Provincial Agricultural Products Quality Safety Center

关键词: Cyclocarya paliurus;    Polysaccharide biosynthesis;    Antioxidant activity;    Industrial production;   

DOI  :  10.7717/peerj.11615

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Cyclocarya paliurus (Batal.) Iljinskaja is a common endemic tree species and used as a Chinese medicine. The main active components in the leaves of this plant are polysaccharides. However, the temporal patterns of gene expression underlying the synthesis of polysaccharides in C. paliurus at different leaf developmental stages and its relationship with the polysaccharide content and antioxidant activities has not been reported to date. Methods RNA-seq was used to investigate the biosynthesis pathway of polysaccharides at the four developmental stages of C. paliurus leaves. The content and the antioxidant activities of polysaccharides were measured with typical biochemical methods and the identified correlations were statistically evaluated. Results Sixty-nine differentially expressed genes were found in the leaves during different developmental stages of C. paliurus. These are associated with glycosyltransferases and belong to 18 families. During different developmental stages of C. paliurus, the polysaccharide content first increased and then decreased, and the UDP-glucose 4-epimerase gene was found to be significantly positively correlated with the polysaccharide content. The clearance rates of DPPH radicals, superoxide anion radicals, hydroxyl radicals, and the reducing power of polysaccharides in the leaves of C. paliurus at different developmental stages showed a dose-dependent relationship with the concentration of polysaccharides. Conclusions The smallest fully expanded leaves are suitable for high-quality tea, and leaves with sizes below the largest fully expanded leaves are suitable for industrial production of polysaccharides.

【 授权许可】

CC BY