Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification

PeerJ

article

Wannapimol Kriangwanich¹  Kittisak Buddhachat²  Anocha Poommouang¹  Siriwadee Chomdej²  Chatchote Thitaram⁵  Patcharaporn Kaewmong⁶  Kongkiat Kittiwattanawong⁶  Korakot Nganvongpanit¹ 

[1] Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University;Excellence Center in Veterinary Bioscience, Chiang Mai University;Department of Biology, Faculty of Science, Naresuan University;Department of Biology, Faculty of Science, Chiang Mai University;Center of Elephant and Wildlife Health, Faculty of Veterinary Medicine, Chiang Mai University;Phuket Marine Biological Center

关键词: Dolphin;    Dugong;    Melting temperature;    ISSR;   

DOI  :  10.7717/peerj.11689

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Currently, species identification of stranded marine mammals mostly relies on morphological features, which has inherent challenges. The use of genetic information for marine mammal species identification remains limited, therefore, new approaches that can contribute to a better monitoring of stranded species are needed. In that context, the ISSR-HRM method we have proposed offers a new approach for marine mammal species identification. Consequently, new approaches need to be developed to identify individuals at the species level. Eight primers of the ISSR markers were chosen for HRM analysis resulting in ranges of accuracy of 56.78–75.50% and 52.14–75.93% in terms of precision, while a degree of sensitivity of more than 80% was recorded when each single primer was used. The ISSR-HRM primer combinations revealed a success rate of 100% in terms of discrimination for all marine mammals included in this study. Furthermore, ISSR-HRM analysis was successfully employed in determining marine mammal discrimination among varying marine mammal species. Thus, ISSR-HRM analysis could serve as an effective alternative tool in the species identification process. This option would offer researchers a heightened level of convenience in terms of its performance and success rate. It would also offer field practice to veterinarians, biologists and other field-related people a greater degree of ease with which they could interpret results when effectively classifying stranded marine mammals. However, further studies with more samples and with a broader geographical scope will be required involving distinct populations to account for the high degree of intraspecific variability in cetaceans and to demonstrate the range of applications of this approach.

【 授权许可】

CC BY