Helicobacter pylori prevalence in healthy Mexican children: comparison between two non-invasive methods

PeerJ

article

Verónica I. Martínez-Santos¹  Manuel Hernández Catalán²  Luis Octavio Ojeda Salazar²  Octavio Andrei Orozco Gómez²  Sandra Ines Lorenzo²  Rayver Santos Gómez³  Norma S. Romero-Castro⁴  Roxana Reyes Ríos⁵  Dinorah Nashely Martinez Carrillo²  Gloria Fernández-Tilapa² 

[1]Cátedras CONACyT-Universidad Autónoma de Guerrero

[2]Laboratorio de Investigación Clínica, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero

[3]Ih Max Gabinete de Diagnóstico, Universidad de Valle de Guerrero

[4]Facultad de Odontología, Universidad Autónoma de Guerrero

[5]Escuela Superior de Ciencias Naturales, Universidad Autónoma de Guerrero

关键词: H. pylori;    Children;    Non-invasive methods;    Mexico;    Prevalence;   

DOI  :  10.7717/peerj.11546

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Helicobacter pylori detection in asymptomatic children with suspected infection or with symptoms that suggest gastric pathology is problematic, since most of the methods depend on the endoscopic study, an invasive and expensive method. Non-invasive methods can be a feasible alternative but must be validated. The purpose of this study was to evaluate the concordance between H. pylori DNA detection in saliva and dental plaque by PCR, with antigen detection in stool by immunochromatography, among asymptomatic children in the state of Guerrero, Mexico. Methods Dental plaque, saliva, and stool samples were obtained from 171 children between 6 and 12 years old. H. pylori detection in saliva and dental plaque was performed by PCR using specific primers for the 16S rRNA gene, while the detection in stool samples was performed by immunochromatography using the CerTest kit. Results We found an overall H. pylori prevalence of 59.6% (102/171). Of the H. pylori positive children 18% (20/111) were positive in saliva samples, 28.1% (34/121) in dental plaque samples, and 50.4% (71/141) in stool samples. A higher prevalence was found in girls (64.7%, p = 0.002). Although some of the children declared some dyspeptic symptoms, these were no related to H. pylori. In conclusion, we found a high prevalence of H. pylori in asymptomatic children and the highest proportion was detected by stool antigen test, which was the most feasible method to detect H. pylori infection.

【 授权许可】

CC BY