MALDI-TOF MS as a method for rapid identification of Phytophthora de Bary, 1876

PeerJ

article

Matěj Božik¹  Marcela Mrázková²  Karolína Novotná¹  Markéta Hrabětová²  Petr Maršik¹  Pavel Klouček¹  Karel Černý² 

[1] Czech University of Life Sciences, Department of Food Science;The Silva Tarouca Research Institute for Landscape and Ornamental Gardening

关键词: Phytophthora;    Maldi;    Biotyping;    Species determination;    ITS;    COI;    Library;    Database;    Mass spectra;    Protein;   

DOI  :  10.7717/peerj.11662

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The number of described species of the oomycete genus Phytophthora is growing rapidly, highlighting the need for low-cost, rapid tools for species identification. Here, a collection of 24 Phytophthora species (42 samples) from natural as well as anthropogenic habitats were genetically identified using the internal transcribed spacer (ITS) and cytochrome c oxidase subunit I (COI) regions. Because genetic identification is time consuming, we have created a complementary method based on by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). Both methods were compared and hypothesis that the MALDI-TOF MS method can be a fast and reliable method for the identification of oomycetes was confirmed. Over 3500 mass spectra were acquired, manually reviewed for quality control, and consolidated into a single reference library using the Bruker MALDI Biotyper platform. Finally, a database containing 144 main spectra (MSPs) was created and published in repository. The method presented in this study will facilitate the use of MALDI-TOF MS as a complement to existing approaches for fast, reliable identification of Phytophthora isolates.

【 授权许可】

CC BY