Hallmarks of glycogene expression and glycosylation pathways in squamous and adenocarcinoma cervical cancer

PeerJ

article

Patricia Martinez-Morales¹  Irene Morán Cruz²  Lorena Roa-de la Cruz³  Paola Maycotte⁴  Juan Salvador Reyes Salinas⁵  Victor Javier Vazquez Zamora⁵  Claudia Teresita Gutierrez Quiroz⁵  Alvaro Jose Montiel-Jarquin⁵  Verónica Vallejo-Ruiz² 

[1] CONACYT-Centro de Investigación Biomédica de Oriente, Mexican Institute of Social Security;Centro de Investigación Biomédica de Oriente, Laboratory of Molecular Biology, Instituto Mexicano del Seguro Social;Department of Biological Chemical Sciences, Universidad de las Américas-Puebla;Centro de Investigación Biomédica de Oriente, Laboratory of Cell Biology, Instituto Mexicano del Seguro Social;Hospital de especialidades, General Manuel Ávila Camacho, Instituto Mexicano del Seguro Social

关键词: Glycogene signature;    Glycome;    Cervical cancer;    Glycosylation;    RNAseq;    Gene expression microarray;    Squamous cervical cancer;    Cervical adenocarcinoma;   

DOI  :  10.7717/peerj.12081

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Dysregulation of glycogene expression in cancer can lead to aberrant glycan expression, which can promote tumorigenesis. Cervical cancer (CC) displays an increased expression of glycogenes involved in sialylation and sialylated glycans. Here, we show a comprehensive analysis of glycogene expression in CC to identify glycogene expression signatures and the possible glycosylation pathways altered. Methods First, we performed a microarray expression assay to compare glycogene expression changes between normal and cervical cancer tissues. Second, we used 401 glycogenes to analyze glycogene expression in adenocarcinoma and squamous carcinoma from RNA-seq data at the cBioPortal for Cancer Genomics. Results The analysis of the microarray expression assay indicated that CC displayed an increase in glycogenes related to GPI-anchored biosynthesis and a decrease in genes associated with chondroitin and dermatan sulfate with respect to normal tissue. Also, the glycogene analysis of CC samples by the RNA-seq showed that the glycogenes involved in the chondroitin and dermatan sulfate pathway were downregulated. Interestingly the adenocarcinoma tumors displayed a unique glycogene expression signature compared to squamous cancer that shows heterogeneous glycogene expression divided into six types. Squamous carcinoma type 5 (SCC-5) showed increased expression of genes implicated in keratan and heparan sulfate synthesis, glycosaminoglycan degradation, ganglio, and globo glycosphingolipid synthesis was related to poorly differentiated tumors and poor survival. Squamous carcinoma type 6 (SCC-6) displayed an increased expression of genes involved in chondroitin/dermatan sulfate synthesis and lacto and neolacto glycosphingolipid synthesis and was associated with nonkeratinizing squamous cancer and good survival. In summary, our study showed that CC tumors are not a uniform entity, and their glycome signatures could be related to different clinicopathological characteristics.

【 授权许可】

CC BY