Downregulation of PPA2 expression correlates with poor prognosis of kidney renal clear cell carcinoma

PeerJ

article

Wenbiao Zhu¹  Huiming Jiang²  Shoucheng Xie¹  Huanqin Xiao¹  Qinghua Liu¹  Nanhui Chen²  Pei Wan²  Shanming Lu¹ 

[1] Department of Pathology, Meizhou People’s Hospital;Department of Urology, Meizhou People’s Hospital

关键词: Kidney renal clear cell carcinoma;    PPA2;    Prognosis;    Biomarker;   

DOI  :  10.7717/peerj.12086

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundKidney renal clear cell carcinoma (KIRC) is the most common subtype of kidney cancer. Inorganic pyrophosphatase (PPA2) is an enzyme that catalyzes the hydrolysis of pyrophosphate to inorganic phosphate; few studies have reported its significance in cancers. Therefore, we aimed to explore the prognostic value of PPA2 in KIRC.MethodsPPA2 expression was detected via immunohistochemistry in a tissue chip containing specimens from 150 patients with KIRC. We evaluated the correlation between PPA2 expression, clinicopathological characteristics, and survival. Data from online databases and another cohort (paraffin-embedded specimens from 10 patients with KIRC) were used for external validation.ResultsPPA2 expression was significantly lower in KIRC tissues than in normal renal tissues (p < 0.0001). Low expression of PPA2 was significantly associated with a high histologic grade and poor prognosis. The differential expression of PPA2 was validated at the gene and protein levels. Multivariate Cox regression analysis showed that PPA2 expression was an independent prognostic factor in patients with KIRC. Gene set enrichment analysis suggested that decreased expression of PPA2 might be related to the epithelial-mesenchymal transition in KIRC.ConclusionsOur study demonstrated that PPA2 is an important energy metabolism-associated biomarker correlated with a favorable prognosis in KIRC.

【 授权许可】

CC BY