Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis

PeerJ

article

Min Liu¹  Fei Mo²  Xiaohan Song³  Yun He²  Yan Yuan³  Jiaoyan Yan³  Ye Yang³  Jian Huang²  Shu Zhang² 

[1] Department of Laboratory Medicine, Sichuan Maternal and Child Health Hospital, Chengdu, Sichuan Province;Department of Clinical Laboratory, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province;Department of Basic Clinical Laboratory Medicine, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang, Guizhou Province

关键词: Breast cancer;    Diagnosis;    Bioinformatics;    Biomarker;    microRNA;    Exosome;   

DOI  :  10.7717/peerj.12147

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

PurposeBreast cancer (BC) is characterized by concealed onset, delayed diagnosis, and high fatality rates making it particularly dangerous to patients’ health. The purpose of this study was to use comprehensive bioinformatics analysis and experimental verification to find a new biomarker for BC diagnosis.MethodsWe comprehensively analyzed microRNA (miRNA) and mRNA expression profiles from the Gene Expression Omnibus (GEO) and screened out differentially-expressed (DE) miRNAs and mRNAs. We used the miRNet website to predict potential DE-miRNA target genes. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID), we performed Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on overlapping potential target genes and DE-mRNAs. The protein-protein interaction (PPI) network was then established. The miRNA-mRNA regulatory network was constructed using Cytoscape and the analysis results were visualized. We verified the expression of the most up-regulated DE-miRNA using reverse transcription and a quantitative polymerase chain reaction in BC tissue. The diagnostic value of the most up-regulated DE-miRNA was further explored across three levels: plasma-derived exosomes, cells, and cell exosomes.ResultsOur comprehensive bioinformatics analysis and experimental results showed that hsa-miR-21-5p was significantly up-regulated in BC tissue, cells, and exosomes. Our results also revealed that tumor-derived hsa-miR-21-5p could be packaged in exosomes and released into peripheral blood. Additionally, when evaluating the diagnostic value of plasma exosomal hsa-miR-21-5p, we found that it was significantly up-regulated in BC patients. Receiver operating characteristic (ROC) analysis also confirmed that hsa-miR-21-5p could effectively distinguish healthy people from BC patients. The sensitivity and specificity were 86.7% and 93.3%, respectively.ConclusionThis study’s results showed that plasma exosomal hsa-miR-21-5p could be used as a biomarker for BC diagnosis.

【 授权许可】

CC BY