Characterizations of novel pesticide-degrading bacterial strains from industrial wastes found in the industrial cities of Pakistan and their biodegradation potential

PeerJ

article

Noreen Asim¹  Mahreen Hassan²  Farheen Shafique⁴  Maham Ali⁶  Hina Nayab⁷  Nuzhat Shafi⁵  Sundus Khawaja⁸  Sadaf Manzoor⁹ 

[1] Division of Genomics and Bioinformatics Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar;Department of Molecular Biology and Biotechnology, University of Sheffield;Microbiology, Shaheed Benazir Bhutto Women University;Department of Biomedical Science, University of Sheffield;Department of Zoology, University of Azad Jammu and Kashmir Muzaffarabad;Department of Zoology, University of Peshawar;Institute of Biological Sciences, Sarhad University of Science and Information Technology;Department of Biotechnology, University of Azad Jammu and Kashmir Muzaffarabad;Department of Statistics, Islamia College University

关键词: Organo-pesticides;    Biodegrading-bacteria;    Molecular characteristics;    Phylogenetic analysis;    16S rRNA;   

DOI  :  10.7717/peerj.12211

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundLack of infrastructure for disposal of effluents in industries leads to severe pollution of natural resources in developing countries. These pollutants accompanied by solid waste are equally hazardous to biological growth. Natural attenuation of these pollutants was evidenced that involved degradation by native microbial communities. The current study encompasses the isolation of pesticide-degrading bacteria from the vicinity of pesticide manufacturing industries.MethodsThe isolation and identification of biodegrading microbes was done. An enrichment culture technique was used to isolate the selected pesticide-degrading bacteria from industrial waste.ResultsAround 20 different strains were isolated, among which six isolates showed significant pesticide biodegrading activity. After 16S rRNA analysis, two isolated bacteria were identified as Acinetobacter baumannii (5B) and Acidothiobacillus ferroxidans, and the remaining four were identified as different strains of Pseudomonas aeruginosa (1A, 2B, 3C, 4D). Phylogenetic analysis confirmed their evolution from a common ancestor. All strains showed distinctive degradation ability up to 36 hours. The Pseudomonas aeruginosa strains 1A and 4D showed highest degradation percentage of about 80% for DDT, and P. aeruginosa strain 3C showed highest degradation percentage, i.e., 78% for aldrin whilst in the case of malathion, A. baumannii and A. ferroxidans have shown considerable degradation percentages of 53% and 54%, respectively. Overall, the degradation trend showed that all the selected strains can utilize the given pesticides as sole carbon energy sources even at a concentration of 50 mg/mL.ConclusionThis study provided strong evidence for utilizing these strains to remove persistent residual pesticide; thus, it gives potential for soil treatment and restoration.

【 授权许可】

CC BY