Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil

PeerJ

article

Shaohua Chu¹  Xiaorui Liu¹  Dan Zhang¹  Xiaotong Chai¹  Xijia Yang¹  Yuee Zhi¹  Yaowei Chi¹  Pei Zhou¹  Bin Wang¹ 

[1] School of Agriculture and Biology, Shanghai Jiao Tong University;Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs;Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University

关键词: Bacillus megaterium NCT-2;    Nitrate;    Microbial community;    FUNGuild;    Ectomycorrhizal fungi;   

DOI  :  10.7717/peerj.12309

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundSecondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communities have not been explored.MethodsGreenhouse experiments were carried out to investigate changes in soil properties, Brassica chinensis L. growth, bacterial, and fungal community structure and function in response to NCT-2 inoculation.ResultsThe NCT-2 inoculant significantly reduced the nitrate content in B. chinensis and inhibited the rebound of soil nitrate in the later stage. The shifts of bacterial community structure and function by NCT-2 was negligible, and a greater disturbance of soil fungal community structure and function was observed, for example the strong inhibitory effect on ectomycorrhizal fungi. These results indicated that the NCT-2 inoculant likely achieved the remediation effect in secondary salinized soil by shifting fungal community. The present findings add to the current understanding of microbial interactions in response to bacterial inoculation and can be of great significance for the application of NCT-2 inoculants in secondary salinized soil remediation.

【 授权许可】

CC BY