Identification of genes and gene expression associated with dispersal capacity in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae)

PeerJ

article

Victor A. Shegelski¹  Maya L. Evenden¹  Dezene P.W. Huber²  Felix A.H. Sperling¹ 

[1] Department of Biological Sciences, University of Alberta;Faculty of Environment, University of Northern British Columbia, Prince George

关键词: Dispersal;    Flight genetics;    Flight mill;    Gene expression;    Forest pest;    Genetic systems;    Dendroctonus;    Dispersal physiology;    Flight physiology;    Insect flight;   

DOI  :  10.7717/peerj.12382

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Dispersal flights by the mountain pine beetle have allowed range expansion and major damage to pine stands in western Canada. We asked what the genetic and transcriptional basis of mountain pine beetle dispersal capacity is. Using flight mills, RNA-seq and a targeted association study, we compared strong-flying, weak-flying, and non-flying female beetles from the recently colonized northern end of their range. Nearly 3,000 genes were differentially expressed between strong and weak flying beetles, while weak fliers and nonfliers did not significantly differ. The differentially expressed genes were mainly associated with lipid metabolism, muscle maintenance, oxidative stress response, detoxification, endocrine function, and flight behavior. Three variant loci, two in the coding region of genes, were significantly associated with flight capacity but these genes had no known functional link to flight. Several differentially expressed gene systems may be important for sustained flight, while other systems are downregulated during dispersal and likely to conserve energy before host colonization. The candidate genes and SNPs identified here will inform further studies and management of mountain pine beetle, as well as contribute to understanding the mechanisms of insect dispersal flights.

【 授权许可】

CC BY