Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations

PeerJ

article

Ozkan Aydemir¹  Benedicta Mensah²  Patrick W. Marsh¹  Benjamin Abuaku³  James Leslie Myers-Hansen²  Jeffrey A. Bailey¹  Anita Ghansah² 

[1] Department of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Brown University;Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana;Department of Epidemiology, Noguchi Memorial Institute for Medical Research, University of Ghana

关键词: Malaria;    Plasmodium falciparum;    Drug resistance;    Molecular inversion probes;    Molecular surveillance;   

DOI  :  10.7717/peerj.11794

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases at the time of diagnostic testing. This streamlined process involving a single tube minimized clinical and laboratory work and provided accurate frequencies of all known drug resistance mutations after high-throughput targeted sequencing using molecular inversion probes. Our study validates this method as a cost-efficient, accurate and highly-scalable approach for drug resistance mutation monitoring that can potentially be applied to other infectious diseases such as tuberculosis.

【 授权许可】

CC BY