Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay

PeerJ

article

Shai Shefer¹  Arthur Robin¹  Alexander Chemodanov¹  Mario Lebendiker²  Robert Bostwick³  Lynn Rasmussen³  Michael Lishner⁴  Michael Gozin⁵  Alexander Golberg¹ 

[1] Port School of Environment and Earth Sciences, Tel Aviv University;Silberman Institute of Life Science, Hebrew University of Jerusalem;Sothern Research;Meir Medical Center;School of Chemisty, Tel Aviv University

关键词: SARS-CoV-2;    Antiviral;    Seaweed;    Macroalgae;    Sulfated polysaccharides;    Ulva sp.;    Extraction;    Ulvan;   

DOI  :  10.7717/peerj.12398

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found in Ulva sp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.

【 授权许可】

CC BY