Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum

PeerJ

article

Zilin Zhao¹  Jiaran Shuang²  Zhaoguo Li²  Huimin Xiao²  Yuling Liu²  Tao Wang²  Yangyang Wei²  Shoulin Hu¹  Sumei Wan¹  Renhai Peng¹ 

[1] College of Plant Science, Tarim University;Anyang Institute of Technology

关键词: Gossypium hirsutum;    Golden2-like;    Gene family;    Phylogenetic analysis;    Abiotic stress;   

DOI  :  10.7717/peerj.12484

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Golden2-Like (GLK) transcription factors are a type of transcriptional regulator in plants. They play a pivotal role in the plant physiological activity process and abiotic stress response. Methods In this study, the potential function of GLK family genes in Gossypium hirsutum was studied based on genomic identification, phylogenetic analysis, chromosome mapping and cis-regulatory elements prediction. Gene expression of nine key genes were analyzed by qRT-PCR experiments. Results Herein, we identified a total of 146 GhGLK genes in Gossypium hirsutum, which were unevenly distributed on each of the chromosomes. There were significant differences in the number and location of genes between the At sub-genome and the Dt sub-genome. According to the phylogenetic analysis, they were divided into ten subgroups, each of which had very similar number and structure of exons and introns. Some cis-regulatory elements were identified through promoter analysis, including five types of elements related to abiotic stress response, five types of elements related to phytohormone and five types of elements involved in growth and development. Based on public transcriptome data analysis, we identified nine key GhGLKs involved in salt, cold, and drought stress. The qRT-PCR results showed that these genes had different expression patterns under these stress conditions, suggesting that GhGLK genes played an important role in abiotic stress response. This study laid a theoretical foundation for the screening and functional verification of genes related to stress resistance of GLK gene family in cotton.

【 授权许可】

CC BY